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. 2019 Dec 18;8(1):4. doi: 10.3390/microorganisms8010004

Figure 1.

Figure 1

Purified Shiga toxin 2k (Stx2k) and Stx2kE167Q. Stx2k and Stx2kE167Q purified from Shiga toxin-producing Escherichia coli (STEC) 309 and BL21-Stx2kE167Q bacterial cells, respectively, were analyzed by Coomassie staining on a 4–12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weights (kDa) of protein markers (M) are indicated next to the marker. The A- and B-subunits are denoted by an arrow at the right side of the panel. Each lane contains 0.5 µg of purified protein.