Table 1.

Strains used in this study.

Strain Name	Protein Expressed	Reference
BL21-stx2kE167Q *	Stx2kE167Q	This study
RM10638 *	Stx2a	[40]
RM7103	n/a	[41]
STEC309 *	Stx2k	[37]
STEC313	Stx2k	[37]
STEC388 a	Stx2k	[7,37]
STEC409	Stx2k, Stx2e	[37]
CWG308 pJCP-Gb3	Gb3-LPS	[42]
CWG308 pJCP-lgtCDE	Gb4-LPS	[43]
CWG308	n/a	[42]

An * indicates the strains were used for protein purification. ^a stx2k gene in strain STEC388 was initially assigned as a Stx2e variant [7], but further analysis reassigned it as a new Stx2k subtype [37].