FIG 1.
Infection of humanized mice with HIV-Vif variants. (A) Newborn NSG mice were irradiated after birth, and human donor cord blood-derived CD34+ cells were transplanted into them. The mice were infected intraperitoneally with 2 × 105 TCID50 of virus (HIV-WT, HIV-45G, or HIV-ΔSLQ). The specifics for each of the three infection experiments are provided in the timelines. Plasma was collected at the indicated time points for viremia measurements and sequence analysis. (B) Comparison of plasma viremias at day 30 postinfection (baseline) in mice infected with different viruses in three different experiments (#1, #2, and #3). The lower limit of detection of the assay was 400 copies (cp)/ml. The means and standard deviations of the viral loads are depicted. Viremia for HIV-ΔSLQ was significantly different from HIV-WT or HIV-45G viremia (P ≤ 0.0020; Mann-Whitney test). **, P ≤ 0.01.