FIG 6.

Characterizing HIV vif mutations in infected mice. (A) Genotypes of vif codon 45 in five mice infected with HIV-45G and treated with 3TC from 30 to 58 days postinfection. The percentages of 45G and revertant E45 sequences are indicated on stacked bar plots. The percentages of the latter are annotated, as well. (B) Dot plots depicting nucleotide diversity (π) in vif sequences in individual mice (data points) infected with HIV-WT or HIV-45G at 30 days postinfection (prior to 3TC treatment). The horizontal lines depict means. (C) Dot plots depicting fractions of GG and GA dinucleotides mutated to AG (GG-to-AG) and AA (GA-to-AA), respectively, in individual mice (data points) at 30 days postinfection. The horizontal lines depict means. (D) Stop codon rates were quantitated across plasma vif sequences at day 30 postinfection (as in Fig. 5B). (E) Dot plots depicting π in vif sequences in individual mice (data points) at day 58 postinfection in 3TC-treated mice. P = 0.0357; Mann-Whitney test. *, P ≤ 0.05. (F) Fractions of GG-to-AG or GA-to-AA dinucleotide mutations in vif at day 58 postinfection in 3TC-treated mice. (G) Stop codon rates in vif sequences determined at day 58 postinfection in 3TC-treated mice.