FIG 3.

ADCC activity of MUC16-eluted IgG. (A and B) Gating strategy for percent cytotoxicity in the positive-control antibody 311.H (A) and MUC16-eluted IgG (B). SSC, side scatter; FSC, forward scatter. (C) The ADCC activity of MUC16-eluted IgG was quantified by a modified RFADCC assay. As a reference, the input IgG and GuHCl-treated IgG from SIV-positive and -negative animals were used. In addition, a positive control, 311.H, and negative controls, 6.10B and Synagis, were used (n = 4 SIV⁺ and 3 SIV⁻ rhesus macaques). Median values are displayed as center bars, with error bars showing the ranges. (D) The area under the curve was calculated under each condition.