FIG 5.

Linear peptide binding to MUC16-eluted IgG. Linear epitope mapping of purified IgG (GuHCl control and MUC16 eluted) against overlapping peptides covering full-length SIVmac239 and SIVsmE550 Envs was performed. Linear epitope regions are indicated along the x axis with amino acid position ranges (by SIVmac239 numbering). The percentage of total Env binding for each epitope is calculated as 100 × the magnitude of binding to each epitope/sum of the magnitude of binding to all epitopes (n = 8 rhesus macaques). *, FDR-corrected P value of <0.05 by a sign test.