FIG 7.

gB_498–505 expressed from gCp results in a delayed gB-CD8⁺ T cell immunodominance in ganglia. Corneas of mice were infected as described previously, with 1 × 10⁵ PFU/eye of HSV-1 WT or recombinant HSV-1 expressing gB_498–505 from the gC promoter. At 8, 10, 12, 16, 21, and 30 dpi, TG were dissociated and stained with antibodies to CD45, CD3, and CD8 and with MHC-I gB_498–505 tetramer, as detailed in Materials and Methods. (A) The total number of TG-infiltrating gB-specific CD8s is shown over time in a gCp infection. (B) The fraction of gB_498–505 tetramer-positive cells among the total CD8⁺ T cells in the TG is shown over time in each infection. Bars represent the mean and standard deviation for each group. *, P < 0.05; **, P < 0.01; ****, P < 0.0001, by unpaired t test between groups at each time point.