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. 2020 Feb 14;94(5):e01384-19. doi: 10.1128/JVI.01384-19

FIG 1.

FIG 1

HUVEC stably expressing vIL-6 have increased ITGB3 mRNA and protein levels. (A) Relative ITGB3 mRNA expression in stable HUVEC normalized to the expression levels in EV-HUVEC. (B) Integrin β3 protein expression in the total cell lysate of stable HUVEC. (C) (Top) Relative luciferase expression from a luciferase reporter under the control of an ITGB3-promoter transfected into HEK293T cells. (Bottom) Immunoblots for vIL-6 and actin from transfected HEK293T cells. (D) Integrin αV protein expression in the total cell lysate of stable HUVEC. (E) Surface expression of αVβ3 integrin in stable HUVEC was measured using flow cytometry. The gray histogram represents EV HUVEC, and the white histogram represents vIL-6 HUVEC. **, P < 0.01; ***, P < 0.001.