FIG 2.

vIL-6 induces ITGB3 expression in a paracrine manner. (A and B) HUVEC were treated with conditioned medium from EV- or vIL-6-expressing HUVEC for 24 h, followed by the comparison of ITGB3 mRNA levels (A) and protein levels (B). (C and D) Similar experiments were conducted using conditioned medium from EV- and vIL-6-expressing BJABs. (E) Conditioned media were collected from EV- and vIL-6-HUVEC in the presence of nonspecific mouse IgG or mouse anti-vIL-6 IgG. This conditioned medium was then placed on HUVEC. After 24 h, lysates were collected, and immunoblotting was performed for actin and ITGB3. CM, conditioned medium; NS, nonspecific. *, P < 0.05; ***, P < 0.001.