FIG 3.

vIL-6 from lytically replicating cells promotes the expression of ITGB3 in a paracrine manner. (A) iSLK.219 cells were reactivated with doxycycline for 24 h, and the levels of ITGB3, vIL-6, and actin were detected by immunoblotting. (B) Lysates from reactivated (24 h post-doxycycline treatment) iSLK.219 and vIL-6-expressing HUVEC were resolved by SDS-PAGE, and vIL-6 expression was detected by immunoblotting. (C) HUVEC were treated for 24 h with conditioned medium from latent or reactivated iSLK.219 (24 h post-doxycycline treatment). Immunoblotting was performed with HUVEC lysates and iSLK.219 conditioned medium. (D) Conditioned media were collected from TREx-Rta-BCBL-1 after 24 h of DMSO (latent) or doxycycline (reactivated [react.]) treatment. Conditioned medium was supplemented with nonspecific mouse IgG or mouse anti-vIL-6 IgG and placed on HUVEC. After 24 h, lysates were collected, and immunoblotting was performed. BCBL-1 cell conditioned medium was processed in the same manner.