FIG 4.

JAK/STAT3 signaling is necessary for vIL-6-induced ITGB3. (A) Whole-cell lysates from EV- and vIL-6-HUVEC were immunoprecipitated with a FLAG (vIL-6) antibody. The eluates and inputs were resolved by SDS-PAGE, and immunoblotting was performed for the indicated proteins. (B) EV- and vIL-6-HUVEC were transfected with siRNAs for 72 h, and lysates were probed for the indicated proteins. (C) EV- and vIL-6-HUVEC were treated with the STAT3 inhibitor cryptotanshinone (0 or 20 μM) for 48 h. Lysates were then collected, and immunoblotting was performed for the indicated proteins. (D) EV- and vIL-6-HUVEC were transfected with siRNAs for 48 h, and lysates were probed for the same proteins as for panel C. (E) HUVEC were transfected with siRNAs against a nontargeting control or STAT3. Twenty-four hours posttransfection, cells were treated with conditioned medium from EV- or vIL-6-HUVEC and incubated for an additional 24 h before lysates were collected and used for immunoblotting. NTC, nontargeting control; H, HUVEC.