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. 2020 Jan 16;10(1):149. doi: 10.3390/biom10010149

Figure 4.

Figure 4

Effects of intracerebroventricular (i.c.v.) injection of methamphetamine (MA) on Fos expression and phosphorylation of GluN1 serine 896 residue in the rostral ventrolateral medulla (RVLM) in urethane-anesthetized Sprague Dawley rats. (A) Top panel shows representative Western blot analysis of the levels of FOS and β-actin in the RVLM 2 h after i.c.v. injection of saline (S), MA 50 nmol (50), and MA 150 nmol (150). The percentage changes in the ratio of FOS to β-actin are shown in the bottom graph. The ratio treatment with saline is taken as a control of 100%. Values represent the mean + SEM, n = 8 animals in each group. * p < 0.05 versus saline analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison post-test. (B) Top panel shows representative Western blot analysis of the levels of phosphoserine 896 on the GluN1 subunit (pGluN1-Ser 896) and GluN1subunit (GluN1) in the RVLM 5 min after i.c.v. injection of saline (S) and MA 150 nmol (150). The percentage changes in the ratio of pGluN1-Ser 896 to GluN1 are shown in the bottom graph. The ratio treatment with saline is taken as a control of 100%. Values represent the mean + SEM, n = 6 animals in each group. * p = 0.0166 versus saline analyzed by unpaired t-test.