Figure 7.

Effects of intracerebroventricular (i.c.v.) injected methamphetamine (MA) on glutamate concentration in the rostral ventrolateral medulla (RVLM) in anesthetized rats. Top panel shows the experimental design for the set of experiments. A dialysis probe was inserted into the RVLM more than 60 min before the first collection of dialysate. At the end, the first collection of dialysate from the RVLM (pre-MA), MA 150 nmol was applied cerebroventricularly through the i.c.v. cannulated tube. The dialysate from the RVLM was collected in 10-min periods after MA was injected (1st, 2nd, and 3rd). Bar graph shows the glutamate concentration in the RVLM dialysate 10-min period before and every 10-min period after i.c.v. injection of 150 nmol MA. Values represent the mean + SEM, n = 5 animals, in the set of tests. # p < 0.0001 versus pre-MA and ^v p < 0.0001 versus 1st 10 min analyzed by one way ANOVA repeated measures followed by Bonferroni’s multiple comparison post-test.