Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers and probes sequences for Rec and Trans real-time PCR.
| Oligonucleotide | Sequence 5′-3′ | Size |
|---|---|---|
| Rec-fwd | AAATAATGCGGAGTTAAAAGGTGAA | 74 bp |
| Rec-rev | TGGACTGCATTTGGTATGTGAGT | |
| Rec-probe | FAM-TACGGATTGCCGTCCCCGAAAGT-BHQ1 | |
| Trans-fwd | CTCATTACGTTGATTGGCATACG | 79 bp |
| Trans-rev | GGTTCGTGGTCTCCTTTTACAATAA | |
| Trans-probe | JOE-AACGAAGAAAAGGGAAAAACTCCCACCC-BHQ1 |
