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Global fit of 1:1 Langmuir binding model with trastuzumab for (a) PG and (b) LPG. Black lines constitute two repeat injections of PG and LPG over trastuzumab immobilized on a surface. The concentrations of each analyte (PG and LPG) injected were 23.1, 11.6, 5.79, 2.89, and 1.45 nM. Experiments were performed over a 700 s period, but for graphical display only the first 450 s are shown. Dissociation was performed for a total of 5 min.
