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. 2019 Dec 18;10(1):4. doi: 10.3390/biom10010004

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a) Experimental workflow to determine the interaction of antibody (trastuzumab) to silica-immobilized LPG. (b) QCM-D response for the binding interactions of trastuzumab to silica-immobilized LPG and subsequent detection of HER2 antigen spiked in 25% human serum. The arrows indicate the time points for the injection of LPG, trastuzumab, and HER2. (c) Frequency shifts for binding interactions between trastuzumab and HER2 spiked in various biological fluids. Extensive PBS washing was performed between each sample injection.

(a) Experimental workflow to determine the interaction of antibody (trastuzumab) to silica-immobilized LPG. (b) QCM-D response for the binding interactions of trastuzumab to silica-immobilized LPG and subsequent detection of HER2 antigen spiked in 25% human serum. The arrows indicate the time points for the injection of LPG, trastuzumab, and HER2. (c) Frequency shifts for binding interactions between trastuzumab and HER2 spiked in various biological fluids. Extensive PBS washing was performed between each sample injection.