Table 3.
Comparison of the viscoelastic parameters for PG immobilization mediated by linker and through chemical conjugation and their subsequent effect on antibody and antigen binding. Each parameter is an average of three independent measurements.
| Immobilization | Thickness of Bound Protein (nm) |
Mass of Protein Deposited (ng/cm2) |
Thickness of Bound Antibody (nm) |
Mass Deposited for Bound Antibody (ng/cm2) |
Thickness of Bound Antigen (nm) |
Mass Deposited for Bound Antigen (ng/cm2) |
|---|---|---|---|---|---|---|
| LPG physisorbed | 7.84 ± 0.22 | 862.95 | 20 ± 0.05 | 1200.14 | 27.84 ± 0.74 | 1600.47 |
| PG chemically immobilized | 2.89 ± 0.18 | 256.76 | 7.43 ± 0.15 | 450.79 | 12.76 ± 0.22 | 578.43 |