Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 23;10(1):11. doi: 10.3390/metabo10010011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Copper induced oxidative stress in porcine small intestinal epithelial (IPEC-J2) cells. Cells were treated with CuSO₄, Cu-Gly, and Cu-Pro at the concentrations of 30 and 120 μM for 10 h. (a) Total superoxide dismutase (SOD) activity was assessed using an assay kit. The result was expressed as a percentage of the control. (b) SOD1 protein expression levels were measured by Western blotting. The densitometry of blot images normalized to β-actin levels for each lane. (c) Reactive oxygen species (ROS) was determined by using 2, 7-dichlorofluorescin diacetate (DCFH-DA). (d) Malondialdehyde (MDA) was measured by an acid reactive substance (TBARS) assay. The result was corrected with the total protein content of cells. Data represent mean values ± standard deviation (n = 3). Significant differences between processing groups are represented by different lowercase letters (p < 0.05).

Copper induced oxidative stress in porcine small intestinal epithelial (IPEC-J2) cells. Cells were treated with CuSO₄, Cu-Gly, and Cu-Pro at the concentrations of 30 and 120 μM for 10 h. (a) Total superoxide dismutase (SOD) activity was assessed using an assay kit. The result was expressed as a percentage of the control. (b) SOD1 protein expression levels were measured by Western blotting. The densitometry of blot images normalized to β-actin levels for each lane. (c) Reactive oxygen species (ROS) was determined by using 2, 7-dichlorofluorescin diacetate (DCFH-DA). (d) Malondialdehyde (MDA) was measured by an acid reactive substance (TBARS) assay. The result was corrected with the total protein content of cells. Data represent mean values ± standard deviation (n = 3). Significant differences between processing groups are represented by different lowercase letters (p < 0.05).