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. 2020 Jan 16;12(1):70. doi: 10.3390/pharmaceutics12010070

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell viability (%) of monolayer HCT116 colon cancer cells in the presence of prepared nanoformulations and free Pip. The cells were treated at different concentrations and incubated for 24 h (A), 48 h (B), and 72 h (C). A red asterisk (*) indicates significant (p < 0.05) differences between nanoformulations and free Pip, whereas a black asterisk (*) indicates significant differences between nanoformulations. NS, not significant. A black asterisks (****) indicate very significant differences. All data are expressed as mean ± SD.