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. 2020 Jan 17;10(1):160. doi: 10.3390/ani10010160

Table 3.

The effect of positioning (rear- and forward-facing and stock) on observed behaviours. Data are expressed as the least square mean and standard error (SE), with p-value determined by linear mixed model and Tukey post-hoc testing. Means with different superscript differ significantly (A, B, C, p < 0.01; a, b, p < 0.05).

Behaviour Rear Facing Forward Facing Stocks p-Value
Backward movement 5.3 ± 4.8 A 15.1 ± 4.9 B 4.3 ± 6.7 <0.001
Biting 2.1 ± 2.2 1.9 ± 2.1 0.1 ± 3.0 ns
Explorative behaviour 2.6 ± 0.8 1.3 ± 0.8 2.1 ± 1.0 ns
Forward movements 3.6 ± 4.7 A 14.5 ± 4.8 B 4.8 ± 6.6 <0.001
Scratching 2.4 ± 0.6 2.0 ± 0.7 1.8 ± 0.6 ns
Head surveying 39.6 ± 4.1 A 36.1 ± 4.3 A 7.8 ± 4.7 B <0.001
Head tossing 9.5 ± 1.4 A 9.2 ± 1.7 A 4.1 ± 1.5 B <0.001
Interaction 6.4 ± 1.3 A,a 12.2 ± 1.4 B 4.1 ± 1.3 A,b 0.000
Lateral movements 26.8 ± 2.1 A 48.9 ± 2.4 B 8.0 ± 2.1 C <0.001
Leaning on the stall rails 56.2 ± 9.2 A 51.1 ±9.3 A 1.2 ± 12.4 B 0.001
Licking 13.5 ±2.7 A 25.9 ± 2.8 B 2.1 ±3.4 C <0.001
Loss of balance 18.6 ± 6.5 A 8.3 ± 6.5 B 0 ± 9.2 <0.001
Pawing 0.9 ± 0.5 0.4 ± 0.6 1.2 ± 0.5 ns
Touching the tie cord 15.4 ± 2.8 A 14.3 ± 3.1 A 0 ± 3.3 B <0.001
Turning the head 28.1 ± 3.4 A 27.9 ± 3.7 A 18.0 ± 3.4 B <0.001
Total behaviour 230.7 ± 30.4 A 269.2 ± 30.7 B 57.8 ±41.7 C <0.001
Total stress 115.2 ± 6.5 A 121.0 ± 7.4 A 34.7 ± 6.5 B <0.001
Total balance 109.4 ± 26.9 A 135.2 ± 27.1 B,a 18.0 ± 37.5 b 0.002