Figure 6.

The estimates of the total cellular glutathione system enzymes activities in the E. magnusii yeast. (A)—glycerol-; (B)—glucose-containing media. * Unit of enzymatic activity per 1 mg of protein. Cellular GPxs were measured at 340 nm in 0.05 mM K-P_i-buffer, pH 7.4; containing 1 mM EDTA, 0.12 mM NADP⁺, 0.85 mM GSH, 0.37 mM H₂O₂, and 1 unit of GR per mL. The same medium without glutathione served as the control. The reaction was initiated by the enzyme application. The rate of NADP⁺ reduction due to enzyme reactions such as oxidized glutathione formation by GPxs action followed by its reduction resulted from NADP⁺ oxidation by GR action was monitored by decrease in A. * One unit of enzyme (EU) activity was defined as the enzyme amount catalyzing 1 µmol final reaction product at +25 °C per a min. The calculation is the same as that for isocitrate dehydrogenase (IDH). Values are mean ± SEM from 5–6 independent experiments. Error bars represent the standard deviation of triplicates to each experiment. a, b—p < 0.05; c, d—p < 0.04.