Table 3.
Viable cell counts and 16S rRNA gene copy number of mono-cultures and co-cultures after 24 h culture in cation adjusted Mueller Hinton (CAMH) medium with or without 28 mM glycerol.
| Strains | Viable Cell Counts (Log CFU/mL) |
16S rRNA Gene Copy Number (Log Gene copies/mL) |
||
|---|---|---|---|---|
| Biphasic CAMH with 28 mM glycerol | Biphasic CAMH without glycerol | Biphasic CAMH with 28 mM glycerol | Biphasic CAMH without glycerol | |
| Mono-cultures | ||||
| C. jejuni N16-1419 | 8.1 ± 0.4 A | 8.4 ± 0.6 A | 9.6 ± 0.3 a | 9.8 ± 0.1 a |
| L. reuteri PTA8_1 | 7.0 ± 0.2 a | 6.6 ± 0.1 a | 7.9 ± 0.1 A | 7.3 ± 0.4 A |
| L. reuteri PTA5_F13 | 4.5 ± 0.4 A | 6.7 ± 0.1 B | 8.0 ± 0.2 a | 7.8 ± 0.2 a |
| Co-cultures * | ||||
| C. jejuni N16-1419 + L. reuteri PTA8_1 | 7.5 ± 0.4 A | 8.5 ± 0.8 A | 9.9 ± 0.2 a | 10.2 ± 0.1 a |
| C. jejuni N16-1419 + L. reuteri PTA5_F13 | BDL a | 9.3 ± 0.1 b | 6.3 ± 0.1 A | 10.2 ± 0.1 B |
Data presented as the mean and standard deviation of three biological replicates. Mean values with different alphabetical superscript letters are significantly different at p < 0.05. * In co-cultures only C. jejuni cells were enumerated on selective plates (CampyFood ID Agar) and qPCR BDL, below the detection limit, with a limit of detection 1 log CFU/mL.