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. 2019 Dec 18;8(1):5. doi: 10.3390/microorganisms8010005

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Tracing of the suspected colonies using rep-PCR analysis. Yogurt with strain 11251 (Leu. mesenteroides) using (GTG)₅ primer (a); lane 1—marker 100 bp, lane 2–16 (colonies Y1–15), lane 17—11251 strain, lane 18—marker 100 bp; lower half of the gel: lane 19—marker 100 bp, lane 20–34 (colonies Y16–30), lane 35—11251 strain, lane 36—marker 100 bp. REP primer (b); and ERIC primer (c); lane 1—marker 1 kb, lane 2—marker 100 bp, lane 3–17 (colonies Y1–15), lane 18—11251 strain, lane 19—marker 100 bp, lane 20—marker 1 kb; lower half of the gel: lane 21—marker 1 kb, lane 22—marker 100 bp, lane 23–37 (colonies Y16–Y30), lane 38—11251 strain, lane 39—marker 100 bp, lane 40—marker 1 kb. Yogurt with strain B151 (L. brevis) using (GTG)₅ primer (d); lane 1—marker 100 bp, lane 2–16 (colonies YB1–15), lane 17—B151 strain, lane 18—marker 100 bp; lower half of the gel: lane 19—marker 100 bp, lane 20–34 (colonies YB16–30), lane 35—B151 strain, lane 36—marker 100 bp. REP primer (e); lane 1—marker 100 bp, lane 2–16 (colonies YB1–15), lane 17—B151 strain, lane 18—marker 100 bp, lane 19—marker 1 kb; lower half of the gel: lane 20—marker 100 bp, lane 21–35 (colonies YB16–YB30), lane 36—B151 strain, lane 37—marker 100 bp, lane 38—marker 1 kb. ERIC primer (f); lane 1—marker 1 kb, lane 2—marker 100 bp, lane 3–17 (colonies YB1–15), lane 18—B151 strain, lane 19—marker 100 bp, lane 20—marker 1 kb; lower half of the gel: lane 21—marker 1 kb, lane 22—marker 100 bp, lane 23–37(colonies YB16–30), lane 38—B151 strain, lane 39—marker 100 bp, lane 40—marker 1 kb. Yogurt with strain LB41^K (L. plantarum) using (GTG)₅ primer (g); REP primer (h); ERIC primer (i); lane 1—marker 1 kb, lane 2—marker 100 bp, lane 3–17 (colonies YP1–15), lane 18—strain LB41^K, lane 19—marker 100 bp, lane 20—marker 1 kb; lower half of the gel: lane 21—marker 1 kb, lane 22—marker 100 bp, lane 23–37 (colonies YP16–30), lane 38—LB41^K strain, lane 39—marker 100 bp, lane 40—marker 1 kb. Positive controls—11251, B151, and LB41^K.