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. 2020 Jan 17;8(1):129. doi: 10.3390/microorganisms8010129

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Phylogenetic tree based on the sequences of the 16S rRNA gene (1498 bp) among 36 species of Aeromonas. (B) Phylogenetic tree based on the concatenated sequences of rpoD and gyrB genes (1098) among the most similar species based on the 16S rRNA gene. The number in the nodes indicates the bootstrap values substitutions estimated by site. (C) Results (%) for the ANI (average nucleotide identity) and isDDH (in silico DNA–DNA hybridization) obtained between the genomes of the most similar species; notice that ANI and isDDH values are ≤96% and ≤70% in all cases, respectively which are the cut-off values established for delimiting Aeromonas spp.