Figure 1.

Winter-acclimated larvae survive freezing and supercooling at −5 °C equally well. (a) Survival of frozen and supercooled larvae following cold exposure at −5 °C for the indicated time periods. (b) Midgut and (c) fat body cell survival following freezing and supercooling at −5 °C for 24 h or 14 days. In all panels, symbols represent mean ± s.e.m., N = 3 for a, N = 5 for b and c. In b and c, different letters indicate statistically significant differences between groups (GLM, planned contrast with Tukey correction, p < 0.05).