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. 2020 Jan 16;10(1):150. doi: 10.3390/biom10010150

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Extracellular vescile (EV) characterization. (A) Nanoparticle tracking analysis (NTA) showing a peak between 100–200 nm for the control (CT), luminal A (LA), and triple negative (TNBC) groups. (B) Transmission electron microscopy (TEM) image of EVs from cancer patient showing a size corresponding to NTA results. Size bar = 200 nm. (C) Western blotting (WB) analysis showing strong protein expression of CD9 and CD63 on EVs from control (2) and cancer (4), when compared with corresponding serum supernatant EV-depleted from control (1) and cancer (3).