Table 3.

Targeted C. elegans genes primers for qPCR analysis.

Gene Name	Gene Type	Forward Primer (5′–3′)	Reverse Primer (5′–3′)	Reference
cdc-42	housekeeping	ATCCACAGACCGACGTGTTT	GTCTTTGAGCAATGATGCGA	[42]
Y45F10D.4	housekeeping	CGAGAACCCGCGAAATGTCGGA	CGGTTGCCAGGGAAGATGAGGC	[43]
daf-2	GOI	AAAAGATTTGGCTGGTCAGAGA	TTTCAGTACAAATGAGATTGTCAGC	[44]
daf-16	GOI	TTCAATGCAAGGAGCATTTG	AGCTGGAGAAACACGAGACG	[44]
sek-1	GOI	GCCGATGGAAAGTGGTTTTA	TAAACGGCATCGCCAATAAT	[44]
pmk-1	GOI	CCGACTCCACGAGAAGGATA	AGCGAGTACATTCAGCAGCA	[44]
abf-2	GOI	TCGTCCGTTCCCTTTTCCTT	CCTCTCTTAATAAGAGCACC	[12]
fipr-22/fipr-23	GOI	CCCAATCCAGTATGAAGTTG	ATTTCAGTCTTCACACCGGA	[12]
cnc-4	GOI	ATGCTTCGCTACATTCTCGT	TTACTTTCCAATGAGCATTC	[12]
cnc-7	GOI	TTTTGTTGGCTCTGGTGGCA	ATGAGTCCAGGACGGTACAT	[12]