Figure 3.

Total myofibrillar, myosin, actin, and sarcoplasmic protein concentrations. Data include total myofibrillar (MF) protein concentrations (panel a), total sarcoplasmic protein (SP) concentrations (panel b), total protein concentrations (panel c), myosin heavy chain protein abundance (panel d), and actin protein abundance (panel e) of younger trained (YT), younger untrained (YU), and older untrained (OU) participants. Numerical values within bars represent mean ± standard deviation values. Panel f contains a representative Coomassie image of a participant from each cohort (duplicate lanes) where myosin heavy chain is represented by band (a), and actin by band (b). Panels (g,h) are regression plots with 95% confidence intervals showing good agreement between actin and myosin heavy chain abundance (determined via SDS-PAGE) versus total myofibrillar protein concentrations (per mg tissue) (determined using BCA assays).