Table 1.

Linearity, sensitivity, precision and accuracy data for the proposed HPLC-PDA method.

Analyte	tR	λ (nm)	Linear Range (µg/mL)	Regression			LOD (µg/mL)	LOQ (µg/mL)	Precision		Recovery (%)
				Calibration Equation	n	r			Intra-Day (RSD, %)	Inter-Day (RSD, %)
NCHA	3.58	325	1.85–110.5	y = 14,045.0x	6	0.9997	0.402	1.217	1.25	1.72	97.0
CHA	6.19	325	2.65–265.30	y = 14,195.0x	7	0.9997	0.397	1.204	1.40	1.75	100.5
CCHA	6.82	325	1.55–105.4	y = 13,934.0x	6	0.9998	0.405	1.227	1.30	1.65	98.4
PB2	7.33	280	1.65–82.30	y = 2399.4x	6	0.9998	0.144	0.436	2.67	3.31	97.4
ECA	7.91	280	1.49–149.40	y = 3367.1x	7	0.9999	0.077	0.233	2.02	2.63	99.2
PC1	8.71	280	1.52–78.30	y = 2299.8x	6	0.9997	0.135	0.403	2.70	3.51	98.6
CAD	9.12	328	1.92–95.80	y = 5251.7x	6	0.9997	0.071	0.220	1.35	1.69	97.5
QPH	9.54	350	2.16–08.20	y = 6398.0x	6	0.9998	0.018	0.054	1.83	2.01	98.6
RT	10.23	350	1.15–114.90	y = 7135.3x	7	0.9998	0.062	0.187	1.30	1.61	99.3
HP	10.49	350	2.51–251.20	y = 9942.0x	7	0.9998	0.129	0.391	1.25	1.63	98.2
IQ	10.85	350	1.39–69.60	y = 9786.2x	6	0.9997	0.047	0.142	1.78	1.94	96.9
QR	11.83	350	1.01–50.60	y = 8929.3x	6	0.9997	0.006	0.017	1.93	2.16	97.1

Codification of the phenolic compounds is given in Figure 1. λ, the wavelength at which quantitative determinations were performed; Parameters of the regression model: y = ax; y, peak area; x, concentration of standards in µg/mL; n, number of data points (concentration levels); r, correlation coefficient; LOD, limit of detection; LOQ, limit of quantitation. Precision data are presented for the lowest analyte concentration tested. Recovery data refer to the spiked levels of the analyte added to the sample of C. bullatus leaves.