Figure 3.

Reversed-phase HPLC separation of fecal ³H-testosterone metabolites of male (A,B) and female (C,D) mice from the morning (A,C) and evening (B,D) injection group. Radioactivity (solid line) of each fraction was determined by liquid scintillation counting. Immunoreactivity (dashed line) was tested with the testosterone enzyme immunoassays. Open triangles mark the approximate elution positions of respective standards (E2-diSO4 = 17β-estradiol-disulfate, E1-G = estrone-glucuronide, E1S = estrone-sulfate, C = cortisol, Cc = corticosterone, A = androstenedione, T = testosterone, ET = epitestosterone).