Table 1.
Effect of WDL-AuNPs on thiobarbituric acid reactive substances (TBARS) and antioxidant levels in the DEHP treated RIN-5F cell lines.
| Enzymes | Normal Cells | DEHP (625 µM) |
DEHP + WDL-AuNPS (40 µg/mL) | DEHP + WDL-AuNPS (80 µg/mL) |
WDL-AuNPS (80 µg/mL) |
|---|---|---|---|---|---|
|
TBARS
(nmol/mg protein) |
1.50 ± 0.10 a | 5.52 ± 0.44 b | 3.36 ± 0.26 c | 2.10 ± 0.17 d | 1.72 ± 0.15 ad |
|
SOD
(U ∗/mg of protein) |
65.01 ± 4.05 a | 23.8 ± 1.9 b | 45.89 ± 4.45 c | 59.10 ± 5.13 a | 62.01 ± 3.85 a |
|
CAT
(U ∗∗/mg of protein) |
1.5 ± 0.10 a | 0.18 ± 0.01 b | 0.72 ± 0.06 c | 0.98 ± 0.08 d | 1.4 ± 0.12 a |
|
GPx
(U# /mg of protein) |
4.2 ± 0.35 a | 1.87 ± 0.15 b | 3.12 ± 0.25 c | 3.5 ± 0.28 c | 3.8 ± 0.29 a |
Value is given as mean ± S.D. for triplicates in each group. Values not sharing a common superscript differ significantly (a–d) at p < 0.05 (DMRT). U * = the concentration of enzyme needed for inhibiting the chromogen released by 50% in 1 m under standard condition. U ** = µmole of hydrogen peroxide degraded/min. U# = µmole of GSH used/min. SOD: Super oxide dismutase activity; CAT: Catalase activity; GSH-px: Glutathione peroxidase activity.