Figure 2.

OG2 mESCs treated by withdrawing 2i/LIF. (a) All groups were cultured in N2B27+2i/LIF for the first 24 hours, and then differentiation was enabled by inhibitor removal for another 48 or 72 hours in the experimental groups, while the control group was cultured in N2B27+2i/LIF for additional 48 hours. Scale bar, 100 μm. (b) RT-qPCR analysis of the expression of three pluripotency factors in wild type. OG2 mESCs treated by withdrawing 2i/LIF. The error bar represents standard error, ^∗∗∗P < 0.001 vs. the control group, n = 2.