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. 2020 Jan 10;9(1):64. doi: 10.3390/antiox9010064

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MH (a) and DMH (b) inhibits colony formation ability by MH and DMH in HCT-116. Cells were treated with different concentrations (0, 8, 16 and 24 mg/mL) of MH or DMH for 48 h. After treatment the cells were seeded at a density of 1000 cells/well for 12 days. The formed colonies were fixed with 70% ethanol and stained with methylene blue for count colonies. The results are expressed as a % of plating efficiency (PE) relative to control. The values are expressed as the mean of three independent experiments ± SD. The different superscript letters (a–d) in each bar indicate significant difference (p < 0.05).