PCR amplification using the optimized protocols for specific detection of F. graminearum (a) F. langshetiae (b), F. fujikuroi (c) F. poae (d), F. sporotrichioides (e) and F. temperatum (f). Lanes 1 and 2: F. graminearum NRRL 28585, NRRL 28436; Lanes 3 and 4: F. langsethiae L.3.1, L.3.2; Lanes 5 and 6: F. fujikuroi ITEM 4092, ITEM 4093; Lanes 7 and 8: F. poae ITEM 6006, ITEM 6007; Lanes 9 and 10: F. sporotrichioides ITEM 550, ITEM 695; Lane 11: F. subglutinans CBS 747.97; Lane 12: F. temperatum CBS 138.287; Lane 13: F. culmorum ITEM 628; Lane 14: F. globosum ITEM 613; Lane 15: F. equiseti VIo1093; Lane 16: F. verticillioides F1-VERT; Lane 17: F. proliferatum F2-PRO; Lane 18: non template control. M: DNA molecular size 1000 bp marker.