Table 1.
Primer sequence and PCR conditions used in this study.
Target Gene | Primer Sequence (5′–3′) | Annealing | Cycles | Product Size |
---|---|---|---|---|
GAPDH | F-AGATCCACAACGGATACATT | 52 °C | 25 cycles | 309 bP |
R-TCCCTCAAGATTGTCAGCA | ||||
SREP1-c | F-GGAGCCATGGATTGCACATT | 58 °C | 28 cycles | 191 bP |
R-AGGAAGGCTTCCAGAGAGGA | ||||
HSL | F-TGCCCAGGAGTGTGTCTGAG | 61 °C | 33 cycles | 313 bP |
R-AGGACACCTTGGCTTGAGCG | ||||
Leptin | F-CCTGTGGCTTTGGTCCTATCTG | 59 °C | 30 cycles | 244 bP |
R-TATGCTTTGCTGGGGTTTTC | ||||
Adiponectin | F-CTCCACCCAAGGAAACTTGT | 52 °C | 28 cycles | 500 bP |
R-CTGGTCCACATTTTTTTCCT | ||||
PK | F-ATTGCTGTGACTGGATCTGC | 52 °C | 28 cycles | 229 bP |
R-CCCGCATGATGTTGGTATAG | ||||
GLUT-2 | F-AAGGATCAAAGCCATGTTGG | 55 °C | 28 cycles | 330 bP |
R-GGAGACCTTCTGCTCAGTGG | ||||
ACO | F-GCCCTCAGCTATGGTATTAC | 53 °C | 28 cycles | 633 bP |
R-AGGAACTGCTCTCACAATGG | ||||
CPT-1 | F-TATGTGAGGATGCTGCTTCC | 52 °C | 28 cycles | 628 bP |
R-CTCGGAGAGCTAAGCTTGCT |