Figure 6.

Host-induced gene silencing of VdAK enhanced resistance against V. dahliae (Vd). (A) Disease symptoms on N. benthamiana at 12 dpi and growth phenotype on PDA of Vd isolated from infected seedlings. (B) Specific siRNA targeted the VdAK position. The siRNA sequence aligned with VdAK at the predicted binding site. Relative expression levels of VdAK (C), VdVA (D), and VdATP6 (E) in RT-qPCR analysis of the recovered mycelium. Mean (± SE) from three independent experiments that differed significantly in Duncan’s test (p <0.05) are indicated by different letters.