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. 2020 Jan 7;10(1):27. doi: 10.3390/metabo10010027

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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In situ matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) distribution and change in phospholipid molecules. (a–h) Statistical results of the relative changes in metabolites, with the relative intensity of each metabolite calculated by the average intensity ratio of the ischemic hemisphere to the contralateral hemisphere. (a) PE (18:0), (b) PE (16:0/22:6), (c) PE (p-18:0/22:6), (d) PE (18:0/22:6), (e) PA (18:0/22:6), (f) PI (18:0), (g) PI (18:0/20:4), (h) PS (18:0/22:6). The spatial resolution of MALDI-MSI images is 200 μm, and the bar scale is 5 mm. Data are expressed as mean ± SD (n = 4), ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. sham group; * p < 0.05, ** p < 0.01 vs. model group. S: Sham; Mo: Model; TQ: 5 mg/kg thymoquinone; ED: 3 mg/kg edaravone.