In situ matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) distribution and change in other metabolic molecules. (a–i) Statistical results of the relative changes in metabolites, with the relative intensity of each metabolite calculated by the average intensity ratio of the ischemic hemisphere to the contralateral hemisphere. (a) Na+, (b) K+, (c) Taurine, (d) Ascorbic acid, (e) Glutathione, (f) Arachidonic acid, (g) Pantetheine 4′-phosphate, (h) Glycerol 3-phosphate, (i) Hippuric acid. The spatial resolution of MALDI-MSI images is 200 μm, and the bar scale is 5 mm. Data are expressed as mean ± SD (n = 4), #
p < 0.05, ##
p < 0.01, ###
p < 0.001 vs. sham group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. model group. S: Sham; Mo: Model; TQ: 5 mg/kg thymoquinone; ED: 3 mg/kg edaravone.