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. 2019 Dec 29;9(1):31. doi: 10.3390/antiox9010031

Figure 9.

Figure 9

A-ITC and/or P-ITC treatments maintain the overexpression of heme oxygenase 1 (HO-1), quinone oxidoreductase-1 (NQO1), glutathione S-transferase mu 1 (GSTM1), and/or glutathione S-transferase alpha 1 (GSTA1) in the hippocampus of mice with osteoarthritis pain. The relative protein levels of (A) HO-1, (B) NQO1, (C) GSTM1, and (D) GSTA1 in the hippocampus of MIA-injected mice treated with A-ITC, P-ITC, or vehicle are presented. The SS-injected mice treated with vehicle were used as controls. (E) Representative blots for HO-1 (32 kDa), NQO1 (28 kDa), GSTM1 (26 kDa), GSTA1 (25 kDa), and GAPDH (37 kDa). All proteins are expressed relative to GAPDH levels. In all panels, * denotes significant differences vs. SS-injected mice treated with vehicle, # vs. MIA-injected mice treated with A-ITC and $ vs. MIA-injected mice treated with P-ITC (p < 0.05; one-way ANOVA and Student–Newman–Keuls test). The results are presented as the mean ± SEM; n = 4 samples per experimental group.