Table 1.
The details of PCR-RFLP method used to identify the polymorphisms in analysed genes.
| Gene | SNP | Gene Region | Endonuclease | Digested PCR Product | refSNP | Primers |
|---|---|---|---|---|---|---|
| SCD | A/G | 3′UTR | HpyCH4III | 72,46,39/111,46 | rs80954581 c.*164A > G |
TGCTACCAGGATGCTAAAGATG CAAGAGAAGGACAATGGGACT |
| ACACA | T/C | 5′UTR | AluI | 126,51,14/101,51, 25,14 | rs325238919 c.-456T > C |
CTCCCCTCTCTCAGCTCCAG GGCAGGAATGTTACAAAACG |
| FASN | G/A | Missense Arg433Gln | HpaII | 208/160 | rs326206604 c.1328G > A |
CTGACCCCAAGCTCTTTGAC CTCACCTTCGAGGTCGCTCT |