Figure 1.

(A) Workflow used for preparing the planktonic (PL) and biofilm (BF) cell cultures of S. aureus ATCC25923. EDBF refers to biofilm culture initiated with planktonic cells at exponential growth phase (initiation culture propagated for 2 h). SDBF refers to biofilm culture initiated with planktonic cells at stationary growth phase (initiation culture propagated for 72 h). (B) Time points of sample withdrawal for surfaceomics and phenotypic analyses. All samples were prepared in biological triplicates. (C) Susceptibility of the PL, EDBF, and SDBF cells at the indicated time points was tested with 400 μM levofloxacin. The error bars denote the standard deviation of the log₁₀-transformed bacterial concentrations from three individual experiments. The difference between the means of treated and nontreated PL8 h cells is statistically significant (a paired t-test). The error bars indicate standard deviation (n =16).