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. 2020 Jan 12;8(1):106. doi: 10.3390/microorganisms8010106

Figure 4.

Figure 4

(A) THP-1-mediated phagocytosis/intra-cellular killing of the fully matured EDBF and SDBF cells at three and 24 hpi. Biofilm cells were detached from the polystyrene support and washed prior to the in vitro test. An unpaired t-test was used to compare the means of both groups. (B) Relative abundances of isaA, isaB, and hlgB specific transcripts in the PL8 h, PL96 h, EDBF_24 h, and SDBF_24 h cells. (C) Relative transcript (squares) versus the protein abundance (bars) levels for IsaA, IsaB, and HlgB in planktonic bacteria at 8 and 96 h time points. (D) Relative transcript (squares) versus protein abundance (bars) levels for IsaB during planktonic and biofilm growth. The error bars in panels B, C, and D indicate standard deviations for ≥2 protein label-free quantification (LFQ) intensity values and relative transcript abundances of two independent ddRT-PCR assays.