Table 1.
Amounts of reagents used (±10%) for the synthesis of folic-thiolated chitosan (FTC) conjugates with different weight ratios of l-cysteine (l-Cys) and results of the number of thiol groups and folic acid (FA) content obtained. EDC—1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide hydrochloride; CHT—chitosan; Fol—folate.
| Conjugate | Chitosan (mg) | l-Cys (mg) | EDC (mM) | CHT:Cys:Fol Ratio (w:w:w) | –SH Total μmol/g Polymer | % Disulfide Bond | Folic Acid μmol/g Polymer |
|---|---|---|---|---|---|---|---|
| FTC1 | 150 | 150 | 50 | 1:1:0.1 | 114.0 ± 5.3 | 65.87 | 5.60 ± 0.28 |
| FTC2 | 150 | 300 | 150 | 1:2:0.1 | 407.5 ± 9.5 | 92.29 | 5.05 ± 0.41 |
| FTC3 | 150 | 600 | 150 | 1:4:0.1 | 141.9 ± 7.0 | 70.71 | 5.11 ± 0.18 |