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. 2020 Jan 16;10(1):146. doi: 10.3390/ani10010146

Table 4.

Molar proportions of volatile fatty acids from the in vitro incubation of E. curvula, lucerne and TMR substrates from E. curvula, lucerne and TMR substrates incubated with stored or fresh plant extracts.

VFA Molar Proportions Extracts Cntrl Mon AV AZ MO TD JA CP SEM p-Values
p S p*S
Acetate TMR Stored 45.5 44.5 33.6 33.9 31.3 31.5 33.5 33.2 1.00 <0.01 0.38 0.47
Fresh 45.5 44.5 34.1 35.3 30.6 29.7 32.5 32.2
Lucerne Stored 43.3 41.9 37.3 34.1 33.1 36.5 32.6 36.1 0.66 <0.01 0.95 0.34
Fresh 43.3 41.9 35.4 35.5 34.1 34.5 34.6 35.6
Eragrostis Stored 44.4 42.2 35.8 36.2 38.2 39.1 38.5 38.1 0.55 <0.01 0.26 0.28
Fresh 44.4 42.0 37.6 36.1 39.0 37.9 34.5 36.8
Propionate TMR Stored 22.7 23.2 20.3 20.2 21.3 19.6 19.7 21.8 0.32 0.04 0.62 0.92
Fresh 22.7 23.2 20.3 19.9 22.6 21.8 18.9 21.6
Lucerne Stored 21.0 19.4 20.8 20.3 19.7 20.6 19.6 20.7 0.13 0.82 0.60 0.14
Fresh 20.1 20.9 19.4 19.7 19.4 20.2 20.7 19.6
Eragrostis Stored 17.9 20.1 21.9 22.1 21.4 21.5 22.5 21.6 0.30 0.01 0.25 0.49
Fresh 17.9 20.1 22.7 21.7 22.9 23.3 21.8 22.6
Iso-butyrate TMR Stored 2.92 3.97 7.66 6.57 6.46 7.42 7.06 9.26 0.43 <0.01 0.79 0.19
Fresh 2.92 3.97 5.80 4.72 6.84 8.63 10.2 9.21
Lucerne Stored 3.00 13.1 8.3 9.59 8.98 7.51 9.16 8.62 0.49 <0.01 0.82 0.86
Fresh 3.00 12.5 9.69 9.14 7.39 7.83 8.51 9.28
Eragrostis Stored 4.89 5.91 12.5 8.97 9.39 9.19 8.63 9.35 0.40 <0.01 0.52 0.10
Fresh 4.89 5.91 7.54 9.26 10.3 8.42 9.96 10.1
Butyrate TMR Stored 12.8 14.5 18.9 19.7 20.3 20.4 19.4 18.5 0.51 <0.01 0.32 0.94
Fresh 12.8 14.6 19.1 20.6 20.7 21.4 18.8 19.5
Lucerne Stored 15.8 10.9 13.5 14.1 14.6 14.2 15.6 13.9 0.27 <0.01 0.68 0.62
Fresh 15.8 10.4 14.1 14.3 14.8 14.7 14.8 14.1
Eragrostis Stored 15.9 12.1 8.72 12.1 11.1 11.4 11.1 11.4 0.40 0.03 0.15 0.18
Fresh 15.6 12.1 14.7 11.6 9.7 11.6 11.6 13.7
Iso-valerate TMR Stored 11.7 9.52 10.3 10.1 11.1 11.3 10.5 11.1 0.16 0.04 0.65 0.99
Fresh 11.7 9.52 1.1 10.6 11.1 11.1 10.5 11.1
Lucerne Stored 12.9 8.16 10.1 10.8 12.1 10.8 12.3 10.3 0.33 0.01 0.60 0.95
Fresh 12.9 12.8 11.3 10.8 12.9 11.8 11.2 11.1
Eragrostis Stored 11.9 10.7 9.91 10.7 9.79 9.14 9.43 9.81 0.24 0.45 0.23 0.81
Fresh 11.9 10.7 9.25 10.9 10.8 11.2 11.5 9.56
Valerate TMR Stored 4.06 4.21 9.13 9.34 9.50 9.82 9.87 6.83 0.43 <0.01 0.21 0.80
Fresh 4.05 4.21 9.6 8.99 8.13 7.51 9.14 6.58
Lucerne Stored 4.76 4.72 10.0 11.1 11.7 10.2 10.6 10.4 0.47 <0.01 0.42 0.76
Fresh 4.76 4.72 10.0 10.4 11.4 10.9 10.1 10.2
Eragrostis Stored 5.22 6.61 11.2 10.0 10.2 9.65 9.86 9.81 0.43 0.03 0.19 0.71
Fresh 5.22 6.61 8.16 10.4 7.64 7.52 11.3 7.61
A: p ratio TMR Stored 1.99 1.92 1.65 1.67 1.46 1.60 1.69 1.52 0.04 <0.01 0.63 0.81
Fresh 1.99 1.92 1.67 1.79 1.36 1.39 1.71 1.48
Lucerne Stored 2.16 2.17 1.78 1.67 1.68 1.76 1.66 1.75 0.03 <0.01 0.74 0.45
Fresh 2.16 2.17 1.82 1.79 1.75 1.71 1.67 1.81
Eragrostis Stored 2.48 2.02 1.63 1.64 1.78 1.82 1.71 1.76 0.05 <0.01 0.03 0.42
Fresh 2.48 2.02 1.65 1.66 1.70 1.63 1.63 1.60

Cntrl, control; Mon, monensin; AV, Aloe vera; AZ, Azadirachta indica; MO, Moringa oleifera; TD, Tithonia diversifolia; JA, Jatropha curcas; CP, Carica papaya; SEM, standard error of mean; p, plant extracts; S, storage; p*S, Interaction between plant extracts and storage.