Table 2.
Primers used for real-time PCR analysis
| Genes | Primers | Primers sequences, 5′ to 3′ | Size, bp | NCBI accession number |
|---|---|---|---|---|
| Lgr5 + | Forward | GCCTTTGTAGGCAACCCTTC | 121 | NM_001315762.1 |
| Reverse | AGGCACCATTCAAAGTCAGTG | |||
| Slc15a1/PepT1 | Forward | CATCGCCATACCCTTCTG | 144 | NM_214347.1 |
| Reverse | TTCCCATCCATCGTGACATT | |||
| Slc7a1/CAT1 | Forward | CAACGACCGGACCAAAACAC | 193 | NM_001012613.1 |
| Reverse | CTGGTACACCATGTTCGGCT | |||
| Slc6a19/B 0 AT1 | Forward | CCTGACGCTTATCAACGGGT | 137 | XM_003359855.4 |
| Reverse | AGTTCATGTCGCAGGTCTGG | |||
| Slc1a1/EAAT3 | Forward | GGCACCGCACTCTACGAAGCA | 177 | NM_001164649.1 |
| Reverse | GCCCACGGCACTTAGCACGA | |||
| Slc2a2/GLUT2 | Forward | GACACGTTTTGGGTGTTCCG | 149 | NM_001097417.1 |
| Reverse | GAGGCTAGCAGATGCCGTAG | |||
| Slc27a4/FATP4 | Forward | AGACACACGTTGGACCTTCC | 188 | XM_021069609.1 |
| Reverse | GCAGGTTGGTGTTGATGAGC | |||
| Chromogranin A | Forward | ACTCCGAGGAGATGAACGGA | 205 | NM_001164005.2 |
| Reverse | CTTGGAGGACGCCTCTTCTG | |||
| Muc2 | Forward | GCTCCAGAGAGAAGGCAGAACC | 171 | XM_021082584.1 |
| Reverse | CTCAGGTGCACAGCGAACTC | |||
| Spp1 | Forward | GCCTCTGCCCTTCCAGTTAAA | 210 | NM_214023.1 |
| Reverse | CTCAGGGCTTTCGTTGGACT | |||
| Trop2 | Forward | CATTACGAGCACCCCACCAT | 239 | XM_003127967.4 |
| Reverse | GTGAGGCGCTTCATGGAGAA | |||
| β-Actin | Forward | AGTTGAAGGTGGTCTCGTGG | 215 | XM_003124280.5 |
| Reverse | TGCGGGACATCAAGGAGAAG |