Fig. 3.

Growth of Coxiella burnetii from tick feces in L-929 cells. a Coxiella-containing vacuoles (indicated by arrows) 19 days after inoculation of L-929 cells with feces of ticks, fed for 36 h on Coxiella-containing blood. The picture was taken at 400× magnification. b Cells were removed from the flask at three different time points and genomic equivalents were determined by quantitative PCR. Samples were measured in duplicate, the mean values and standard deviation are indicated. The data were normalized for the mouse housekeeping gene rpl22