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. 2020 Feb 14;20:51. doi: 10.1186/s12935-020-1136-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Elevated TRIP6 expression is associated with poor prognosis in human breast cancer patients. a Real-time PCR and western blot analysis of TRIP6 expression in immortalized human breast epithelial cell line (MCF-10a) and five breast cancer cell lines. b Real-time PCR and western blot analysis of TRIP6 expression in six matched breast cancer tissues (T) and adjacent noncancerous tissues (ANT). TRIP6 mRNA levels were normalized to that of GAPDH. TRIP6 protein levels were normalized to GAPDH. c The expression of TRIP6 in normal breast tissues and breast cancer tissues at different clinical stages. d Kaplan–Meier survival analysis of the correlation between TRIP6 expression level and 5-year OS in breast cancer patients. e Relapse-free survival (RFS) of breast cancer patients with low versus high TRIP6 expression. f Kaplan–Meier analysis of the RFS in breast cancer using Kaplan–Meier Plotter (http://kmplot.com/analysis/)