Table 7.

Transcript level comparison of P. aeruginosa genes between qRT-PCR and NGS. qRT-PCR is the mean of two biological replicates with three technical replicates for each gene. Reference gene (nadB): L-aspartate oxidase, uvrD, and sodM were downregulated at 24 h with no change at 6 h; fumC1 was upregulated at 6 h and downregulated at 24 h; rpsL was upregulated at 6 h with NC at 24 h exposure.

Genes ID	Gene symbol	NGS		qRT-PCR		Primers	Length (bp)	Description
		Fold change		Fold change
		6 h	24 h	6 h	24 h	5′-sequence-3′
PA5443	uvrD	NC	−3.412 ± 0	NC	−2.54 ± 0.01	GTGCGCCTGTCCAATAC	17	DNA helicase II
						GCCTTCGAAGTTGAGGATAG	20
PA4468	sodM	NC	−3.955 ± 0	NC	−2.44 ± 0.01	GAGCAGCCGGTGGAAAGTCT	20	Superoxide dismutase
						GCGACATCACGGTCCAGAAC	20
PA4470	fumC1	3.618 ± 0	−4.599 ± 0	3.48 ± 0.69	−5.39 ± 0	TCGGGCAACTTCGAACTGAA	20	Fumarate hydratase
						GAGCTTGCCCTGGTTGACCT	20
PA4268	rpsL	2.56 ± 0	NC	3.53 ± 0.53	NC	CGGCACTGCGTAAGGTATGC	20	30S ribosomal protein S12
						CCCGGAAGGTCCTTTACACG	20
PA0761∗	nadB		Reference gene			CTACCTTTATACCAGCAATCCC	22	L-aspartate oxidase
						CGGTGATGAGGAAACTCTTG	20