Fig. 4.

TLR4 elimination increased the level of SYN and PSD-95 following B2M treatment. The intensity of synaptophysin and PSD-95 in the hippocampus were determined at 7 and 28 days after B2M or vehicle treatment (n = 6/subgroup). The relative intensity in arbitrary units compared to β-actin (a). B2M treatment decreased the levels of synaptophysin and PSD-95 were at 7 (b, d) and 28 days (c, e), which was improved in TLR4-KO mice. The data were analyzed using two-way ANOVAs used Tukey’s multiple comparisons test. Values are presented as the means ± SD. Significant differences are expressed as follows: *p < 0.05 compared B2M group vs. C group in WT and TLR4-KO mice, according to the two-way ANOVA; ^#p < 0.05 compared B2M group in TLR4-KO mice vs. WT mice, according to the two-way ANOVA. SYN = synaptophysin, WT = wild type C57BL/6, TLR4-KO = TLR4 knockout