Fig. 8.

TLR4 elimination inhibited the B2M-induced activation of TLR4/MyD88/NF-κB signaling pathway in cultured primary neurons. TLR4 elimination inhibited the activation of TLR4/MyD88/NF-κB signaling pathway after B2M-treatment in cultured primary neurons. (a-c) Expression of TLR4, MyD88 and NF-κB mRNA in cultured primary neurons. (d-g) Western blot analysis of TLR4, MyD88 and NF-κB protein in cultured primary neurons, β-actin was used as an internal control (n = 3/subgroup). The data were analyzed using two-way ANOVAs used Tukey’s multiple comparisons test. Values are presented as the means ± SD. Significant differences are expressed as follows: *p < 0.05 compared B2M group vs. Veh group in WT and TLR4-KO mice, according to the two-way ANOVA; ^#p < 0.05 compared B2M group in TLR4-KO mice vs. WT mice, according to the two-way ANOVA. WT = wild type C57BL/6